plaque forming unit,PFU

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问题 plaque forming unit,PFU

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答案Fhe plaque assay is the most widely used assay for infectious virus.monolayers ofhost cells are moculated with suitabel dilutions of virusand after adsorption are overlaid with medium containingagar or carboxyme thyicellulose to prevent virus spreadingthroughout the culture.after several days,the cells initallyinfected have produced virus that spreads only to sur--rounding cells,producing a small area of infection orplaque.under controlled conditipns,a single plaque canarise form a single infectious virus particle,termed aplaque-forming unit.

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